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Exogenous Wt-p53 Protein Is Active in Transformed Cells but Not in Their Non-transformed Counterparts: Implications for Cancer Gene Therapy Without Tumor Targeting by Gabriella D'Orazi; Alessandra Marchetti; Marco Crescenzi; Sabrina Coen; Ada Sacchi; Silvia Soddu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Exogenous Wt-p53 Protein Is Active in Transformed Cells but Not in Their Non-transformed Counterparts: Implications for Cancer Gene Therapy Without Tumor Targeting about?

## Background: Expression of exogenous wild-type p53 (wt-p53) protein in tumor cells can suppress the transformed phenotype whereas it does not apparently induce detrimental effects in non-transformed cells. this observation may provide a molecular basis for p53-mediated gene therapy of p53-sensitive cancers without the need for tumor targeting. ## Methods: To understand the molecular mechanisms responsible for this different behavior in tumor versus normal cells, biochemical and functional analyses of exogenous wt-p53 protein were performed on non-transformed c2c12 myoblasts and their transformed counterparts, the c2-ras cells. ## Results: The exogenous wt-p53 protein, which induced persistent growth arrest only in transformed c2-ras cells, was shown to be significantly more stable in transformed than in non-transformed cells. this different stability was due to different p53 proteolytic degradation. moreover, constitutively, exogenous wt-p53 protein was found to be transcriptionally active only in c2-ras cells but it could also be activated in c2c12 cells by genotoxic damage. ## Conclusions: Non-transformed c2c12 cells present regulatory system(s) which control the expression and

Who reads Exogenous Wt-p53 Protein Is Active in Transformed Cells but Not in Their Non-transformed Counterparts: Implications for Cancer Gene Therapy Without Tumor Targeting?

Author

Gabriella D'Orazi; Alessandra Marchetti; Marco Crescenzi; Sabrina Coen; Ada Sacchi; Silvia Soddu

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)