About this Medicine article
An amplification-free hybridization-based DNA assay to detect Streptococcus pneumoniae utilizing the up-converting phosphor technology by Michel Zuiderwijk; Hans J. Tanke; R. Sam Niedbala; Paul L.A.M. Corstjens is a Medicine article available to read on EtoBox.
## Objectives: Development of a generally applicable sensitive hybridization-based assay devoid of any target amplification for the detection and identification of (pathogenic) bacterial and viral species. ## Design and methods: Using a sandwich hybridization format, the presence of a species-specific nucleic acid sequence is detected by means of lateral flow (lf) and up-converting phosphor technology (upt, a luminescent tracer). as a model, detection of the pathogen streptococcus pneumoniae was investigated using a probe against the single-copy lyta gene. ## Results: Detection of s. pneumoniae (in particular a 1200 p lyta sequences) required less than 1 ng of genomic dna (approximate size 2.2 mb). hybridization and detection were performed in a complex background containing 10 microg fish sperm dna. ## Conclusions: The results indicate the possibility to detect nucleic acid targets in nonamplified dna samples using easy, inexpensive, amplification-free hybridization-based assays and the ultra sensitive upt reporters. employment of upt allows to by-pass target amplification and therefore brings genetic-based testing a step closer to the point-of-care environment. detection of s. pn
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- Author
- Michel Zuiderwijk; Hans J. Tanke; R. Sam Niedbala; Paul L.A.M. Corstjens
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0009-9120)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)