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Atan1p—an extracellular tannase from the dimorphic yeast Arxula adeninivorans : molecular cloning of the ATAN1 gene and characterization of the recombinant enzyme by Erik Böer; Rüdiger Bode; Hans‐Peter Mock; Michael Piontek; Gotthard Kunze is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract The tannase‐encoding __Arxula adeninivorans__ gene __ATAN1__ was isolated from genomic DNA by PCR, using as primers oligonucleotide sequences derived from peptides obtained after tryptic digestion of the purified tannase protein. The gene harbours an ORF of 1764 bp, encoding a 587‐amino acid protein, preceded by an N‐terminal secretion sequence comprising 28 residues. The deduced amino acid sequence was similar to those of tannases from __Aspergillus oryzae__ (50% identity), __A. niger__ (48%) and putative tannases from __A. fumigatus__ (52%) and __A. nidulans__ (50%). The sequence contains the consensus pentapeptide motif (–Gly–X–Ser–X–Gly–) which forms part of the catalytic centre of serine hydrolases. Expression of __ATAN1__ is regulated by the carbon source. Supplementation with tannic acid or gallic acid leads to induction of __ATAN1__, and accumulation of the native tannase enzyme in the medium. The enzymes recovered from both wild‐type and recombinant strains were essentially indistinguishable. A molecular mass of ∼320 kDa was determined, indicating that the native, glycosylated tannase consists of four identical subunits. The enzyme has a temperature optimum at

Who reads Atan1p—an extracellular tannase from the dimorphic yeast Arxula adeninivorans : molecular cloning of the ATAN1 gene and characterization of the recombinant enzyme?

Author

Erik Böer; Rüdiger Bode; Hans‐Peter Mock; Michael Piontek; Gotthard Kunze

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); John Wiley & Sons Inc.; [Chichester]; [New York]: John Wiley & Sons, ©1985-; Wiley; Hindawi Limited (ISSN 0749-503X)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)