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Metabolic Analysis of Mouse Bone-marrow-derived Dendritic Cells Using an Extracellular Flux Analyzer by Kazuhito Gotoh; Yurie Takata; Yuya Nakashima; Soichi Mizuguchi; Keishi Komori; Dongchon Kang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Metabolic Analysis of Mouse Bone-marrow-derived Dendritic Cells Using an Extracellular Flux Analyzer about?
## Summary Dendritic cell (DC) maturation induced by Toll-like receptor (TLR) agonists requires the activation of downstream metabolic changes. Here, we provide a detailed protocol to measure glycolysis, mitochondrial respiration, and fatty acid oxidation in mouse bone-marrow-derived DCs with the Seahorse XF24 extracellular flux (XF) analyzer. XF analysis with the Seahorse bioanalyzer has become a standard method to measure bioenergetic functions in cells, and this protocol can be adapted to other immune cells. For complete information on using this protocol, please refer to Gotoh et al. (2018).
Who reads Metabolic Analysis of Mouse Bone-marrow-derived Dendritic Cells Using an Extracellular Flux Analyzer?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Kazuhito Gotoh; Yurie Takata; Yuya Nakashima; Soichi Mizuguchi; Keishi Komori; Dongchon Kang
- Publisher
- Elsevier BV
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)