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What is pH Impact on Amylase Activity about?

This experiment tested the effect of pH on the activity of the enzyme amylase. Amylase breaks down starch, which can be measured by the transmittance of a solution after iodine, which stains starch, is added. Solutions with pH levels of 3, 5, 7 and 9 were tested over 45 minutes. Results showed that pH 3 had no increase in transmittance, indicating no amylase activity. pH 7 had the highest transmittance, showing it was optimal for amylase activity. pH 5 and 9 showed some activity but less than pH 7, with pH

Author

Pedro Pepe Perez Munoz Lopez

Language

EN