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Protonless ^13^C direct detection NMR: Characterization of the 37 kDa trimeric protein CutA1 by Ivano Bertini; Beatriz Jiménez; Roberta Pierattelli; Anthony G. Wedd; Zhiguang Xiao is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Protonless ^13^C direct detection NMR: Characterization of the 37 kDa trimeric protein CutA1 about?
## Abstract The major limitation of nuclear magnetic resonance spectroscopy arises from the increase of nuclear transverse relaxation rates with increasing molecular mass. This causes reduction in spectral resolution and coherence transfer efficiency. The use of ^2^H‐labeling to eliminate ^1^H‐mediated relaxation pathways and the constructive use of cross correlation effects (TROSY, CRINEPT) alleviate the phenomenon. An alternative approach is to use direct detection of heteronuclei. Specifically designed ^13^C direct detection experiments can complement the set of ^1^H‐based NMR experiments commonly used for structure determination providing an additional source of information less affected by the detrimental transverse relaxation effect. We applied this novel methodology to the study of the CutA1 protein (12.3 kDa) from __E. coli__ that forms a homotrimer in solution with a total molecular mass of 37 kDa. In this work we demonstrate that the information available from ^13^C direct detection experiments makes it possible to completely assign the NMR resonances of the backbone of this 37 kDa trimeric protein without the need of deuteration. The structural and dynamical knowledge ob
Who reads Protonless ^13^C direct detection NMR: Characterization of the 37 kDa trimeric protein CutA1?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ivano Bertini; Beatriz Jiménez; Roberta Pierattelli; Anthony G. Wedd; Zhiguang Xiao
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 0887-3585)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)