Can I read Human cytomegalovirus UL76 induces chromosome aberrations on EtoBox?

Human cytomegalovirus UL76 induces chromosome aberrations by Voon-Kwan Siew; Chang-Yih Duh; Shang-Kwei Wang is a Medicine article available to read on EtoBox.

What is Human cytomegalovirus UL76 induces chromosome aberrations about?

## Abstract ## Background Human cytomegalovirus (HCMV) is known to induce chromosome aberrations in infected cells, which can lead to congenital abnormalities in infected fetuses. HCMV UL76 belongs to a conserved protein family from herpesviruses. Some reported roles among UL76 family members include involvement in virulence determination, lytic replication, reactivation of latent virus, modulation of gene expression, induction of apoptosis, and perturbation of cell cycle progression, as well as potential nuclease activity. Previously, we have shown that stable expression of UL76 inhibits HCMV replication in glioblastoma cells. ## Methods To examine chromosomal integrity and the DNA damage signal γ-H2AX in cells constitutively expressing UL76, immunofluorescent cell staining and Western blotting were performed. The comet assay was employed to assess DNA breaks in cells transiently expressing UL76. ## Results We report that stably transfected cells expressing UL76 developed chromosome aberrations including micronuclei and misaligned chromosomes, lagging and bridging. In mitotic cells expressing UL76, aberrant spindles were increased compared to control cells. However, cells with sup

Who reads Human cytomegalovirus UL76 induces chromosome aberrations?

Author

Voon-Kwan Siew; Chang-Yih Duh; Shang-Kwei Wang

Publisher

BioMed Central; Springer (Biomed Central Ltd.); BioMed Central Ltd.; [Basel Switzerland]: Karger; London: 2009-; Springer Science and Business Media LLC; S. Karger AG; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1021-7770)

Published

2009

Language

EN

Field

Medicine (Life Sciences)