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Quantitative AP-1 gene regulation by oxidative stress in the human retinal pigment epithelium by Edward Chaum; Jinggang Yin; Huaitao Yang; Fridtjof Thomas; John C. Lang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Quantitative AP-1 gene regulation by oxidative stress in the human retinal pigment epithelium about?

## Abstract The purpose of this study was to characterize the early molecular responses to quantified levels of oxidative stress (OS) in the human retinal pigment epithelium (RPE). Confluent ARPE‐19 cells were cultured for 3 days in defined medium to stabilize gene expression. The cells were exposed to varying levels of OS (0–500 μM H~2~O~2~) for 1–8 h and gene expression was followed for up to 24‐h after OS. Using real‐time qPCR, we quantified the expression of immediate early genes from the AP‐1 transcription factor family and other genes involved in regulating the redox status of the cells. Significant and quantitative changes were seen in the expression of six AP‐1 transcription factor genes, __FosB__, __c‐Fos__, __Fra‐1__, __c‐Jun__, __JunB__, and __ATF3__ from 1–8 h following OS. The peak level of induced transcription from OS varied from 2‐ to 128‐fold over the first 4 h, depending on the gene and magnitude of OS. Increased transcription at higher levels of OS was also seen for up to 8‐h for some of these genes. Protein translation was examined for 24‐h following OS using Western blotting methods, and compared to the qPCR responses. We identified six AP‐1 family genes that d

Who reads Quantitative AP-1 gene regulation by oxidative stress in the human retinal pigment epithelium?

Author

Edward Chaum; Jinggang Yin; Huaitao Yang; Fridtjof Thomas; John C. Lang

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)