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Simultaneous refolding, purification, and immobilization of recombinant ** Fibrobacter succinogenes ** 1,3‐1,4‐β‐D‐glucanase on artificial oil bodies by Yi‐Fang Zeng; Ying‐Jing Hung; Ming‐Ju Chen; Chi‐Chung Peng; Jason T. C. Tzen; Je‐Ruei Liu is a Engineering article available to read on EtoBox.
What is Simultaneous refolding, purification, and immobilization of recombinant ** Fibrobacter succinogenes ** 1,3‐1,4‐β‐D‐glucanase on artificial oil bodies about?
## Abstract **BACKGROUND:** 1,3‐1,4‐β‐D‐glucanase (1,3‐1,4‐β‐D‐glucan 4‐glucanohydrolase; EC 3.2.1.73) has been used in a range of industrial processes. As a biocatalyst, it is better to use immobilized enzymes than free enzymes, therefore, the immobilization of 1,3‐1,4‐β‐D‐glucanase was investigated. **RESULTS:** A 1,3‐1,4‐β‐D‐glucanase gene from __Fibrobacter succinogenes__ was overexpressed in __Escherichia coli__ as a recombinant protein fused to the N terminus of oleosin, a unique structural protein of seed oil bodies. With the reconstitution of the artificial oil bodies (AOBs), refolding, purification, and immobilization of active 1,3‐1,4‐β‐D‐glucanase was accomplished simultaneously. Response surface modeling (RSM), with central composite design (CCD), and regression analysis were successfully applied to determine the optimal temperature and pH conditions of the AOB‐immobilized 1,3‐1,4‐β‐D‐glucanase. The optimal conditions for the highest immobilized 1,3‐1,4‐β‐D‐glucanase activity (7.1 IU mg^−1^ of total protein) were observed at 39 °C and pH 8.8. Furthermore, AOB‐immobilized 1,3‐1,4‐β‐D‐glucanase retained more than 70% of its initial activity after 120 min at 39 °C, and it
Who reads Simultaneous refolding, purification, and immobilization of recombinant ** Fibrobacter succinogenes ** 1,3‐1,4‐β‐D‐glucanase on artificial oil bodies?
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- Author
- Yi‐Fang Zeng; Ying‐Jing Hung; Ming‐Ju Chen; Chi‐Chung Peng; Jason T. C. Tzen; Je‐Ruei Liu
- Publisher
- Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0268-2575)
- Published
- 2009
- Language
- EN
- Field
- Engineering (Physical Sciences)