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A perforated patch‐clamp study of calcium currents and exocytosis in chromaffin cells of wild‐type and α~1A~ knockout mice by Marcos Aldea; Kisun Jun; Hee-Sup Shin; Eva Andrés-Mateos; Luisa M. Solís-Garrido; Carmen Montiel; Antonio G. García; Almudena Albillos is a Neuroscience article available to read on EtoBox.
What is A perforated patch‐clamp study of calcium currents and exocytosis in chromaffin cells of wild‐type and α~1A~ knockout mice about?
## Abstract Simultaneous recordings of inward whole‐cell Ca^2+^ channel currents (I~Ca~) and increments of capacitance as an indication of exocytosis (ΔCm), were performed in voltage‐clamped single adrenal chromaffin cells from wild‐type and α~1A~ subunit deficient mice, using the perforated‐patch configuration of the patch‐clamp technique. Using protocol #1 (one single Ca^2+^ channel blocker per cell), to dissect the components of I~Ca~, L channels contributed 43%, N channels 35% and P/Q channels 30% to the total I~Ca~ of wild‐type cells. Using protocol #2 (cumulative sequential addition of 3 μm nifedipine, 1 μmω‐conotoxin GVIA, and 1 μmω‐agatoxin IVA), L, N and P/Q channels contributed 40%, 34% and 14%, respectively, to I~Ca~; an R component of around 11% remained. In wild‐type mice the changes of ΔCm paralleled those of I~Ca~. In α~1A~ deficient mice the L component of I~Ca~ rose to 53% while the P/Q disappeared; the N and R components were similar. In these mice, ΔCm associated to N and R channels did not vary; however, the P/Q component was abolished while the L component increased by 20%. In conclusion, exocytosis was proportional to the relative density of each Ca^2+^ channe
Who reads A perforated patch‐clamp study of calcium currents and exocytosis in chromaffin cells of wild‐type and α~1A~ knockout mice?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Marcos Aldea; Kisun Jun; Hee-Sup Shin; Eva Andrés-Mateos; Luisa M. Solís-Garrido; Carmen Montiel; Antonio G. García; Almudena Albillos
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)
- Published
- 2002
- Language
- EN
- Field
- Neuroscience (Life Sciences)