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Can I read Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility on EtoBox?
Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility by Caleb A. Lareau; Vincent Liu; Christoph Muus; Samantha D. Praktiknjo; Lena Nitsch; Pauline Kautz; Katalin Sandor; Yajie Yin; Jacob C. Gutierrez; Karin Pelka; Ansuman T. Satpathy; Aviv Regev; Vijay G. Sankaran; Leif S. Ludwig is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility about?
Natural sequence variation within mitochondrial DNA (mtDNA) contributes to human phenotypes and may serve as natural genetic markers in human cells for clonal and lineage tracing. We recently developed a single-cell multi-omic approach, called 'mitochondrial single-cell assay for transposase-accessible chromatin with sequencing' (mtscATAC-seq), enabling concomitant high-throughput mtDNA genotyping and accessible chromatin profiling. Specifically, our technique allows the mitochondrial genome-wide inference of mtDNA variant heteroplasmy along with information on cell state and accessible chromatin variation in individual cells. Leveraging somatic mtDNA mutations, our method further enables inference of clonal relationships among native ex vivo-derived human cells not amenable to genetic engineering-based clonal tracing approaches. Here, we provide a step-by-step protocol for the use of mtscATAC-seq, including various cellprocessing and flow cytometry workflows, by using primary hematopoietic cells, subsequent single-cell genomic library preparation and sequencing that collectively take ~3-4 days to complete. We discuss experimental and computational data quality control metrics and
Who reads Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Caleb A. Lareau; Vincent Liu; Christoph Muus; Samantha D. Praktiknjo; Lena Nitsch; Pauline Kautz; Katalin Sandor; Yajie Yin; Jacob C. Gutierrez; Karin Pelka; Ansuman T. Satpathy; Aviv Regev; Vijay G. Sankaran; Leif S. Ludwig
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)