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A Novel Immunocytochemical Assay for the Detection of Serotonin in Platelets by Elisabeth Maurer-Spurej; Kate Dyker; William A. Gahl; Dana V. Devine is a Medicine article available to read on EtoBox.

What is A Novel Immunocytochemical Assay for the Detection of Serotonin in Platelets about?

**Summary.** A method for the rapid, inexpensive and easy detection of platelet serotonin (5‐hydroxytryptamine, 5‐HT) is not currently available. Consequently, many patients suffering from unresolved platelet‐related bleeding disorders are not examined for a possible platelet 5‐HT deficiency. The direct measurement of 5‐HT concentration with high‐performance liquid chromatography (HPLC) or serotonin enzyme‐linked immunosorbent assay (ELISA) is costly and highly demanding. Indirect methods, which determine the content of ATP or calcium with lumi‐aggregometry or electron microscopy, rely upon the assumption that the ATP or calcium concentration is equivalent to that of 5‐HT. We have developed a fluorescence‐based assay for 5‐HT that can be performed within 2 h on fresh or frozen samples using a fluorescence microscope or a flow cytometer. The assay requires only 0·2 ml of platelet‐rich plasma and might therefore be of particular interest for paediatric patients. Samples from control and patient donors were analysed for 5‐HT with the new immunocytochemical assay in comparison with HPLC and/or 5‐HT ELISA. Patients with Hermansky–Pudlak syndrome were readily identified. The new assay wa

Who reads A Novel Immunocytochemical Assay for the Detection of Serotonin in Platelets?

Author

Elisabeth Maurer-Spurej; Kate Dyker; William A. Gahl; Dana V. Devine

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0007-1048)

Published

2002

Language

EN

Field

Medicine (Health Sciences)