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This document describes an experiment to isolate and characterize proteins from a bacterial culture using three objectives: (1) Isolate proteins from bacteria using centrifugation and resuspension, (2) Quantify the extracted protein concentration using spectrophotometry and a Bradford assay, and (3) Separate and visualize the proteins using SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). The results showed the absorbance increased linearly with increasing protein standard concentration
- Author
- Brilliant Siema
- Language
- EN