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Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Formation from γ-Aminobutyrate and Glutamate by Henry E. Valentin; Steven Reiser; Kenneth J. Gruys is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Formation from γ-Aminobutyrate and Glutamate about?

To provide 4-hydroxybutyryl-CoA for poly(3hydroxybutyrate-co-4-hydroxybutyrate) formation from glutamate in Escherichia coli, an acetyl-CoA:4hydroxybutyrate CoA transferase from Clostridium kluyveri, a 4-hydroxybutyrate dehydrogenase from Ralstonia eutropha, a ␥-aminobutyrate:2-ketoglutarate trans- aminase from Escherichia coli, and glutamate decarboxylases from Arabidopsis thaliana or E. coli were cloned and functionality tested by expression of single genes in E. coli to verify enzymatic activity, and uniquely assembled as operons under the control of the lac promoter. These operons were independently transformed into E. coli CT101 harboring the runaway replication vector pJM9238 for polyhydroxyalkanoate (PHA) production. Plasmid pJM9238 contains the PHA biosynthetic operon of R. eutropha under tac promoter control. Polyhydroxyalkanoate formation was monitored by nuclear magnetic resonance (NMR) spectroscopic analysis of the chloroform extracted and ethanol precipitated polyesters. Functionality of the biosynthetic pathway for copolymer production was demonstrated through feeding experiments using various carbon sources that supplied different precursors within the 4HB-CoA biosyn

Who reads Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Formation from γ-Aminobutyrate and Glutamate?

Author

Henry E. Valentin; Steven Reiser; Kenneth J. Gruys

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2000

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)