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Transient RNA interference in hematopoietic progenitors with functional consequences by Daniela M. Oliveira; Margaret A. Goodell is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Summary: Short interfering (si) RNAs have now been shown to inhibit gene expression in several species, including mammals (Elbashir __et al.__: Nature 411:494–498, 2001; Fire __et al.__: Nature 391:806–811, 1998). RNA inhibition in primary cells such as stem cells would facilitate rapid gene discovery in a postgenome era. While retroviruses can deliver siRNA expression cassettes for stable expression (Barton and Medzhitov: Proc Natl Acad Sci USA 99:14943–14945, 2002; Paddison __et al.__: Proc Natl Acad Sci USA 99:1443–1448, 2002; Rubinson __et al.__: Nat Genet 33:401–406, 2003), an efficient method for direct transfer of siRNA to stem cells is still lacking. Here, we established electroporation to deliver siRNA to hematopoietic progenitors. On average, at least 80% of cells take up the RNA, and these display nearly 100% knockout of marker gene expression at both the RNA and protein level. Moreover, knockdown of the hematopoietic regulator, CD45, results in 3‐fold more hematopoietic colonies in a progenitor assay. These results demonstrate that transient transfection of siRNA to primary cells can have substantial functional consequences. This technology may be applicable

Who reads Transient RNA interference in hematopoietic progenitors with functional consequences?

Author

Daniela M. Oliveira; Margaret A. Goodell

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1526-954X)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)