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Development and Application of a Comprehensive Two-dimensional Gas Chromatography with Time-of-flight Mass Spectrometry Method for the Analysis of L-β-methylamino-alanine in Human Tissue by Laura R. Snyder; Jamin C. Hoggard; Thomas J. Montine; Robert E. Synovec is a Chemistry article available to read on EtoBox.
What is Development and Application of a Comprehensive Two-dimensional Gas Chromatography with Time-of-flight Mass Spectrometry Method for the Analysis of L-β-methylamino-alanine in Human Tissue about?
l--Methylamino-alanine (BMAA) has been proposed as a worldwide contributor to neurodegenerative diseases, including Parkinson dementia complex (PDC) of Guam and Alzheimer's disease (AD). Recent conflicting reports of the presence of this amino acid in human brain from patients affected by these diseases have made it necessary to develop methods that provide unambiguous detection in complex samples. Comprehensive two-dimensional gas chromatography coupled with time-of-flightmass-spectrometry analysis (GC × GC-TOFMS) followed by a targeted Parallel Factor Analysis (PARAFAC) deconvolution method has been used recently in metabolomic investigations to separate, identify, and quantify components of complex biological specimens. We have extended and applied this methodology to the toxicological problem of detecting BMAA in extracts of brain tissue. Our results show that BMAA can be isolated from closely eluting compounds and detected in trace amounts in extracts of brain tissue spiked with low levels of this analyte, ranging from 2.5 ppb to 50 ppb, with a limit of detection (LOD) of 0.7 ppb. This new method was sufficiently sensitive to detect BMAA in cerebral extracts of mice fed BMAA.
Who reads Development and Application of a Comprehensive Two-dimensional Gas Chromatography with Time-of-flight Mass Spectrometry Method for the Analysis of L-β-methylamino-alanine in Human Tissue?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Laura R. Snyder; Jamin C. Hoggard; Thomas J. Montine; Robert E. Synovec
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2010
- Language
- EN
- Field
- Chemistry (Physical Sciences)