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Anti-proliferative activity of Artemisia marschalliana on cancerous cell lines by Haleh Forouhandeh; Vahideh Tarhriz; Masoumeh Zadehkamand; Parina Asgharian is a Medicine article available to read on EtoBox.
What is Anti-proliferative activity of Artemisia marschalliana on cancerous cell lines about?
## Background The genus __Artemisia__ of the __Asteraceae__ family has different species that are used in the treatment of a wide range of diseases, including cancers due to the presence of valuable compounds and important medicinal properties. Various studies on the anti-tumor effect of different species of __Artemisia__ have proven the cytotoxic properties of these plants in cancer treatment, and several anti-cancer compounds of this genus have been purified. ## Objective The objective of this study was to investigate the cytotoxicity and related mortality mechanisms of __Artemisia marschalliana__ essential oil and extracts. ## Methods The essential oil and various extracts of __Artemisia marschalliana__ were elicited using a Soxhlet extractor. Anti-cancer to anti-proliferative activity as MTT assay is measuring cancerous and non-cancerous cell viability. In the next step, the strongest extract fractions were obtained by using the vacuum liquid chromatography method. Flow cytometry was applied to identify the mechanism of cell death, and a Real-time polymerase chain reaction test of apoptosis genes, which encode apoptosis-regulating proteins, was measured to confirm the flow cyto
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- Author
- Haleh Forouhandeh; Vahideh Tarhriz; Masoumeh Zadehkamand; Parina Asgharian
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Medicine (Health Sciences)