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Experimental keratitis induced in rat by Acanthamoeba from distinct morphological groups/genotypes: a histological and immunohistochemical evaluation by Norberto de Souza Fernandes; Marcelo Vidigal Caliari; Fabricio Marcos Silva Oliveira; Alexandre Batista Costa Neto; Isabela Aurora Rodrigues; Cinthia Furst; Adriana Oliveira Costa is a Immunology and Microbiology article available to read on EtoBox.
What is Experimental keratitis induced in rat by Acanthamoeba from distinct morphological groups/genotypes: a histological and immunohistochemical evaluation about?
Species of the genus Acanthamoeba are free-living protozoans that occasionally act as parasites, causing a severe, progressive corneal infection termed Acanthamoeba keratitis (AK). The variable pathogenic potential among Acanthamoeba lineages has been shown by in vitro assays, but little is known about the behavior of different strains in animal models of AK. This work aimed to evaluate the infectivity of Acanthamoeba from distinct morphological groups and genotypes in a rat model of AK and apply an immunohistochemical technique for histological characterization of the lesions. Only a strain classified as group I/genotype T17, isolated from a soil source, caused ulcerated corneal lesions in two Wistar rats (n = 9) subjected to intrastromal inoculation. Two strains derived from AK human cases (group II/genotype T4 and group III/genotype T5) did not induce corneal lesions in the rats. A previous association of group II/genotype T4 trophozoites with lethally irradiated Escherichia coli did not influence the infectivity. A hyperimmune serum produced in Wistar rats was validated by an immunocytochemical technique using the three distinct strains and then applied for immunohistochemistry
Who reads Experimental keratitis induced in rat by Acanthamoeba from distinct morphological groups/genotypes: a histological and immunohistochemical evaluation?
It is typically read by researchers, students, and practitioners in Immunology and Microbiology.
- Author
- Norberto de Souza Fernandes; Marcelo Vidigal Caliari; Fabricio Marcos Silva Oliveira; Alexandre Batista Costa Neto; Isabela Aurora Rodrigues; Cinthia Furst; Adriana Oliveira Costa
- Publisher
- Springer Science and Business Media LLC
- Published
- 2023
- Language
- EN
- Field
- Immunology and Microbiology (Life Sciences)