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Cytosine Base Editor 4 but Not Adenine Base Editor Generates Off-target Mutations in Mouse Embryos by Lee, Hye Kyung; Smith, Harold E.; Liu, Chengyu; Willi, Michaela; Hennighausen, Lothar is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Cytosine Base Editor 4 but Not Adenine Base Editor Generates Off-target Mutations in Mouse Embryos about?

## Abstract Deaminase base editing has emerged as a tool to install or correct point mutations in the genomes of living cells in a wide range of organisms. However, the genome-wide off-target effects introduced by base editors in the mammalian genome have been examined in only one study. Here, we have investigated the fidelity of cytosine base editor 4 (BE4) and adenine base editors (ABE) in mouse embryos using unbiased whole-genome sequencing of a family-based trio cohort. The same sgRNA was used for BE4 and ABE. We demonstrate that BE4-edited mice carry an excess of single-nucleotide variants and deletions compared to ABE-edited mice and controls. Therefore, an optimization of cytosine base editors is required to improve its fidelity. While the remarkable fidelity of ABE has implications for a wide range of applications, the occurrence of rare aberrant C-to-T conversions at specific target sites needs to be addressed.

Who reads Cytosine Base Editor 4 but Not Adenine Base Editor Generates Off-target Mutations in Mouse Embryos?

Author

Lee, Hye Kyung; Smith, Harold E.; Liu, Chengyu; Willi, Michaela; Hennighausen, Lothar

Publisher

Springer Science and Business Media LLC; [London]: Springer Nature, 2018-; Society for Mining, Metallurgy and Exploration Inc. (ISSN 2399-3642)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)