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Production of sorbitol and gluconic acid by permeabilized cells of Zymomonas mobilis by Bert Rehr; Cornelia Wilhelm; Hermann Sahm is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Zymomonas mobilis is able to convert glucose and fructose to gluconic acid and sorbitol. The enzyme, glucose-fructose oxidoreductase, catalysing the intermolecular oxidation-reduction of glucose and fructose to gluconolactone and sorbitol, was formed in high amounts [1.4 units (U).mg -1] when Z. mobilis was grown in chemostats with glucose as the only carbon source under non-carbon-limiting conditions. The activity of a gluconolactone-hydrolysing lactonase was constant at 0.2 U.mg-1. Using glucose-grown cells for the conversion of equimolar fructose and glucose mixtures up to 60% (w/v), a maximum product concentration of only 240 g. 1-1 of sorbitol was found. The gluconic acid accumulated was further metabolized to ethanol. After permeabilizing the cells using cationic detergents, maximum sorbitol and gluconic acid concentrations of 295 g. 1 -1 each were reached; no ethanol production occurred. In a continuous process with tccarrageenan-immobilized and polyethylenimin-hardened, permeabilized cells no significant decrease in the conversion yield was observed after 75 days. The specific production rates for a high yield conversion (>98%) in a continuous two-stage process were 0.19 g

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Author

Bert Rehr; Cornelia Wilhelm; Hermann Sahm

Publisher

Springer; Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1432-0614)

Published

1991

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)