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Supercritical Fluid Extraction of Microbial Phospholipid Fatty Acids from Activated Sludge by Muhammad Hanif; Yoichi Atsuta; Koichi Fujie; Hiroyuki Daimon is a Chemistry article available to read on EtoBox.

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Supercritical carbon dioxide (scCO 2 ) extraction was applied for the determination of microbial phospholipid fatty acids (PLFA) in activated sludge. Quantification was performed by using gas chromatography-mass spectrometry (GC-MS). The highest extraction yields of PLFA, at a concentration of 7.28 nmol/mg-dry activated sludge, was obtained at a temperature of 80 • C, pressure of 25 MPa and 10% (v/v) methanol for a 15-min extraction time. ScCO 2 extraction results obtained in these conditions were comparable with those obtained by liquid organic solvent extraction (LSE) based on diversity and equalibility indices. The repeatability test showed that the relative standard deviation values were less than 13%. The experimental results show that the scCO 2 extraction saves time and uses much less organic solvent. In addition, scCO 2 extraction is a promising and alternative method for the analysis of microbial community structure in environmental assessment using the PLFA profile.

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Author

Muhammad Hanif; Yoichi Atsuta; Koichi Fujie; Hiroyuki Daimon

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2010

Language

EN

Field

Chemistry (Physical Sciences)