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Revealing of sugar utilization systems in Halomonas sp. YLGW01 and application for poly(3-hydroxybutyrate) production with low-cost medium and easy recovery by Ye-Lim Park; Hun-Suk Song; Tae-Rim Choi; Sun Mi Lee; Sol Lee Park; Hye Soo Lee; Hyun-Joong Kim; Shashi Kant Bhatia; Ranjit Gurav; Kyungmoon Park; Yung-Hun Yang is a Materials Science article available to read on EtoBox.
What is Revealing of sugar utilization systems in Halomonas sp. YLGW01 and application for poly(3-hydroxybutyrate) production with low-cost medium and easy recovery about?
Poly(3-hydroxybutyrate) (PHB) is a common polyhydroxyalkanoate (PHA) with potential as an alternative for petroleum-based plastics. Previously, we reported a new strain, Halomonas sp. YLGW01, which hyperproduces PHB with 94% yield using fructose. In this study, we examined the PHB production machinery of Halomonas sp. YLGW01 in more detail by deep-genome sequencing, which revealed a 3,453,067-bp genome with 65.1% guanine-cytosine content and 3054 genes. We found two acetyl-CoA acetyltransferases (Acetoacetyl-CoA thiolase, PhaA), one acetoacetyl-CoA reductase (PhaB), two PHB synthases (PhaC1, PhaC2), PHB depolymerase (PhaZ), and Enoyl-CoA hydratase (PhaJ) in the genome, along with two fructose kinases and fructose transporter systems, including the phosphotransferase system (PTS) and ATP-binding transport genes. We then examined the PHB production by Halomonas sp. YLGW01 using high-fructose corn syrup (HFCS) containing fructose, glucose, and sucrose in sea water medium, resulting in 7.95 ± 0.11 g/L PHB (content, 67.39 ± 0.34%). PHB was recovered from Halomonas sp. YLGW01 using different detergents; the use of Tween 20 and SDS yielded micro-sized granules with high purity. Overall, t
Who reads Revealing of sugar utilization systems in Halomonas sp. YLGW01 and application for poly(3-hydroxybutyrate) production with low-cost medium and easy recovery?
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- Ye-Lim Park; Hun-Suk Song; Tae-Rim Choi; Sun Mi Lee; Sol Lee Park; Hye Soo Lee; Hyun-Joong Kim; Shashi Kant Bhatia; Ranjit Gurav; Kyungmoon Park; Yung-Hun Yang
- Publisher
- Elsevier BV
- Published
- 2020
- Language
- EN
- Field
- Materials Science (Physical Sciences)