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MitoSegNet: Easy-to-use Deep Learning Segmentation for Analyzing Mitochondrial Morphology by Fischer, Christian A.; Besora-Casals, Laura; Rolland, Stéphane G.; Haeussler, Simon; Singh, Kritarth; Duchen, Michael; Conradt, Barbara; Marr, Carsten is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is MitoSegNet: Easy-to-use Deep Learning Segmentation for Analyzing Mitochondrial Morphology about?

While the analysis of mitochondrial morphology has emerged as a key tool in the study of mitochondrial function, efficient quantification of mitochondrial microscopy images presents a challenging task and bottleneck for statistically robust conclusions. Here, we present Mitochondrial Segmentation Network (MitoSegNet), a pretrained deep learning segmentation model that enables researchers to easily exploit the power of deep learning for the quantification of mitochondrial morphology. We tested the performance of MitoSegNet against three feature-based segmentation algorithms and the machine-learning segmentation tool Ilastik. MitoSegNet outperformed all other methods in both pixelwise and morphological segmentation accuracy. We successfully applied MitoSegNet to unseen fluorescence microscopy images of mitoGFP expressing mitochondria in wild-type and mutant adults. Additionally, MitoSegNet was capable of accurately segmenting mitochondria in HeLa cells treated with fragmentation inducing reagents. We provide MitoSegNet in a toolbox for Windows and Linux operating systems that combines segmentation with morphological analysis.

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Author

Fischer, Christian A.; Besora-Casals, Laura; Rolland, Stéphane G.; Haeussler, Simon; Singh, Kritarth; Duchen, Michael; Conradt, Barbara; Marr, Carsten

Publisher

Elsevier ; Elsevier Inc.; [Cambridge MA]: Cell Press Elsevier Inc.; Elsevier BV (ISSN 2589-0042)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)