Can I read Identification and analysis of macrophage-derived foam cells from human atherosclerotic lesions by using a “mock” FL3 channel in flow cytometry on EtoBox?

Identification and analysis of macrophage-derived foam cells from human atherosclerotic lesions by using a “mock” FL3 channel in flow cytometry by Yani Liu-Wu; Anders Svenningsson; Sten Stemme; Jan Holm; Olov Wiklund is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Identification and analysis of macrophage-derived foam cells from human atherosclerotic lesions by using a “mock” FL3 channel in flow cytometry about?

Macrophages are one of the major cell types in atherosclerotic lesions. They are believed to play an important role in the pathogenesis and development of the lesion, but their functional state and phenotypic characteristics are not well understood. Using flow cytometry, we analyzed surface markers of macrophages extracted from tissue digests. However, conventional techniques were hampered by the abundance of cell debris and extracellular lipids, which co-localized with double-positive cells in all fluorescent plots. We therefore developed a method to overcome this problem by using a novel gating technique in multiparameter flow cytometry. This method utilized the third fluorescence channel (FL3) as a "mock" channel, since no antibody conjugated to an FL3-specific fluorochrome was added to the samples. Cells single-positive for macrophage-specific monoclonal antibodies (mAb) conjugated to phycoerythrin (PE) (FL2) were separated from non-specific fluorescent particles in the FL2 versus FL3 fluorescent plot and a region excluding debris could be set. This was then used as a gate to exclude debris also in the first fluorescence channel (FL1) vs. FL2 plot in which expression of a panel

Who reads Identification and analysis of macrophage-derived foam cells from human atherosclerotic lesions by using a “mock” FL3 channel in flow cytometry?

Author

Yani Liu-Wu; Anders Svenningsson; Sten Stemme; Jan Holm; Olov Wiklund

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; John Wiley & Sons Ltd.; Wiley (ISSN 0196-4763)

Published

1997

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)