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Bone Marrow Mononuclear Cells Protect Neurons and Modulate Microglia in Cell Culture Models of Ischemic Stroke by Sushil Sharma; Bing Yang; Roger Strong; XiaoPei Xi; Miranda Brenneman; James C. Grotta; Jaroslaw Aronowski; Sean I. Savitz is a Neuroscience article available to read on EtoBox.
What is Bone Marrow Mononuclear Cells Protect Neurons and Modulate Microglia in Cell Culture Models of Ischemic Stroke about?
## Abstract Although several studies have provided evidence for the therapeutic potential of bone marrow‐derived mononuclear cells (MNCs) in animal models of stroke, the mechanisms underlying their benefits remain largely unknown. We have determined the neuroprotective potential of MNCs in primary neuronal cultures exposed to various injuries in vitro. Cortical neurons in culture were exposed to oxygen‐glucose deprivation, hypoxia, or hydrogen peroxide, and cell death was assayed by MTT, caspase‐3 activation or TUNEL labelling at 24 hrs. Cultures were randomized to cotreatment with MNC‐derived supernatants or media before injury exposure. In separate experiments, macrophage or microglial cultures were exposed to lipopolypolysacharide (LPS) in the presence and absence of MNC‐derived supernatants. Neuronal cultures were then exposed to conditioned media derived from activated macrophages or microglia. Cytokines from the supernantants of MNC cultures exposed to normoxia or hypoxia were also estimated by enzyme‐linked immunosorbant assay (ELISA). MNC‐derived supernatants attenuated neuronal death induced by OGD, hypoxia, hydrogen peroxide, and conditioned macrophage/microglial media an
Who reads Bone Marrow Mononuclear Cells Protect Neurons and Modulate Microglia in Cell Culture Models of Ischemic Stroke?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Sushil Sharma; Bing Yang; Roger Strong; XiaoPei Xi; Miranda Brenneman; James C. Grotta; Jaroslaw Aronowski; Sean I. Savitz
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0360-4012)
- Published
- 2010
- Language
- EN
- Field
- Neuroscience (Life Sciences)