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Metalloglycomics of tris(2,2′-bipyridyl) cobalt and ruthenium compounds 1 1 Introduction 1 2 Experimental 2 2.1 Materials 2 2.2 Interaction of metal complexes with FPX 2 2.2.1 Mass spectrometry 2 2.2.2 Isothermal titration calorimetry 2 2.2.3 Methylene blue reporter method 2 2.2.4 1H NMR spectroscopy 2 2.2.5 Circular dichroism spectroscopy 2 2.3 FPX enzymatic cleavage studies 3 2.3.1 Heparinase II from F. heparinum (HepII) 3 2.3.2 Human heparanase (HPSE) 3 3 Results and discussion 3 3.1 FPX binding assays 3 3.2 Assessing the inhibition of FPX cleavage by the enzyme Heparinase II from Flavobacterium heparinum 5 3.3 Assessing the inhibition of FPX cleavage by the human heparanase enzyme (HPSE) 6 4 Conclusions 6 Declaration of Competing Interest 6 Acknowledgements 7 Appendix A Supplementary data 7 References 7
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