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Can I read Bacillus Anthracis Genome Organization in Light of Whole Transcriptome Sequencing on EtoBox?
Bacillus Anthracis Genome Organization in Light of Whole Transcriptome Sequencing by Jeffrey Martin; Wenhan Zhu; Karla D Passalacqua; Nicholas Bergman; Mark Borodovsky is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Bacillus Anthracis Genome Organization in Light of Whole Transcriptome Sequencing about?
## Abstract Emerging knowledge of whole prokaryotic transcriptomes could validate a number of theoretical concepts introduced in the early days of genomics. What are the rules connecting gene expression levels with sequence determinants such as quantitative scores of promoters and terminators? Are translation efficiency measures, e.g. codon adaptation index and RBS score related to gene expression? We used the whole transcriptome shotgun sequencing of a bacterial pathogen __Bacillus anthracis__ to assess correlation of gene expression level with promoter, terminator and RBS scores, codon adaptation index, as well as with a new measure of gene translational efficiency, average translation speed. We compared computational predictions of operon topologies with the transcript borders inferred from RNA-Seq reads. Transcriptome mapping may also improve existing gene annotation. Upon assessment of accuracy of current annotation of protein-coding genes in the __B. anthracis__ genome we have shown that the transcriptome data indicate existence of more than a hundred genes missing in the annotation though predicted by an __ab initio__ gene finder. Interestingly, we observed that many pseudog
Who reads Bacillus Anthracis Genome Organization in Light of Whole Transcriptome Sequencing?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jeffrey Martin; Wenhan Zhu; Karla D Passalacqua; Nicholas Bergman; Mark Borodovsky
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central, [2000]-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2105)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)