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Characterization of the upstream mouse Cbfa1/Runx2 promoter by Z. S. Xiao; Shi-Guang Liu; T. K. Hinson; L. D. Quarles is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Characterization of the upstream mouse Cbfa1/Runx2 promoter about?

## Abstract __Cbfa1 (or Runx2/__AML‐3__/PEPB2α)__ is a transcriptional activator of osteoblastic differentiation. To investigate the regulation of __Cbfa1__ expression, we isolated and characterized a portion of the 5′‐flanking region of the __Cbfa1__ gene containing its “bone‐related” or P1 promoter and exon 1. We identified additional coding sequence in exon 1 and splice donor sites that potentially give rise to a novel __Cbfa1__ isoform containing an 18 amino acid insert. In addition, primer extension mapping identified in the __Cbfa1__ promoter a minor mRNA start site located ∼0.8 kb 5′ upstream of the ATG encoding the MASN/p57 isoform and ∼0.4 kb upstream of the previously reported start site. A luciferase reporter construct containing 1.4 kb of the mouse __Cbfa1__ promoter was analyzed in Ros 17/2.8 and MC3T3‐E1 osteoblast cell lines that express high levels of __Cbfa1__ transcripts. The activity of this construct was also examined in non‐osteoblastic Cos‐7 and NIH3T3 cells that do not express __Cbfa1__ and mesenchymal‐derived cell lines, including CH3T101/2, C2C12, and L929 cells, that express low levels of mature __Cbfa1__ transcripts. The 1.4 kb 5′ flanking sequence of the

Who reads Characterization of the upstream mouse Cbfa1/Runx2 promoter?

Author

Z. S. Xiao; Shi-Guang Liu; T. K. Hinson; L. D. Quarles

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2001

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)