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Can I read A novel sequence element is involved in the transcriptional regulation of expression of the ERG1 (squalene epoxidase) gene in Saccharomyces cerevisiae on EtoBox?
A novel sequence element is involved in the transcriptional regulation of expression of the ERG1 (squalene epoxidase) gene in Saccharomyces cerevisiae by Regina Leber; Rainer Zenz; Klarissa Schröttner; Sandra Fuchsbichler; Brigitte Pühringer; Friederike Turnowsky is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A novel sequence element is involved in the transcriptional regulation of expression of the ERG1 (squalene epoxidase) gene in Saccharomyces cerevisiae about?
Squalene epoxidase is an essential enzyme in the ergosterol‐biosynthesis pathway. It catalyzes the epoxidation of squalene to 2,3‐oxidosqualene and is the specific target of the antifungal drug terbinafine. Treatment of yeast cells with this inhibitor leads to squalene accumulation and sterol depletion. As ergosterol fulfils several essential functions, each requiring optimal sterol concentrations, synthesis of sterols in yeast must be tightly regulated. This study focuses on the sterol‐mediated regulation of expression of the __ERG1__ gene, which codes for squalene epoxidase in __Saccharomyces cerevisiae__. Inhibition of ergosterol biosynthesis with terbinafine increases the expression of __ERG1__ in a concentration‐dependent manner to a maximum of sevenfold. Inhibition of later steps in the ergosterol‐biosynthetic pathway by ketoconazol, an inhibitor of the lanosterol‐14α‐demethylase, and U18666A, an inhibitor of the squalene‐2,3‐epoxide–lanosterol cyclase, also induce expression of __ERG1__, suggesting that __ERG1__ expression is positively regulated by diminished intracellular ergosterol levels. The regulatory effect of sterols is manifested at the level of transcription. Delet
Who reads A novel sequence element is involved in the transcriptional regulation of expression of the ERG1 (squalene epoxidase) gene in Saccharomyces cerevisiae?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Regina Leber; Rainer Zenz; Klarissa Schröttner; Sandra Fuchsbichler; Brigitte Pühringer; Friederike Turnowsky
- Publisher
- John Wiley and Sons; Springer; Wiley (Blackwell Publishing); Springer-Verlag; Blackwell Publishing Inc.; Springer Verlag; Wiley; Springer Science and Business Media LLC (ISSN 1432-1327)
- Published
- 2001
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Physical Sciences)