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The Effects of Removing Dead Bacteria by Propidium Monoazide on the Profile of Salivary Microbiome by Qidi Ren; Fangqiao Wei; Chao Yuan; Ce Zhu; Qian Zhang; Junkang Quan; Xiangyu Sun; Shuguo Zheng is a Dentistry article available to read on EtoBox.

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## Abstract ## Background Oral microbiome played an important role in maintaining healthy state and might exhibit certain changes under circumstances of diseases. However, current microbiological research using sequencing techniques did not regard dead bacteria as a separate part, causing findings based on subsequent analyses on dynamic equilibrium and functional pathways of microbes somewhat questionable. Since treatment by propidium monoazide (PMA) was able to remove dead bacteria effectively, it would be worth studying how the sequencing results after PMA treatment differed from those focusing on the whole microbiota. ## Methods Unstimulated whole saliva samples were obtained from 18 healthy people from 3 age groups (children, adults, and the elderly). After removal of dead bacteria by propidium monoazide (PMA), changes in the profile of salivary microbiome were detected using 16S rRNA sequencing technology, and differences among age groups were compared subsequently. ## Results Dead bacteria accounted for nearly a half of the whole bacteria flora in saliva, while freezing had little effect on the proportion of deaths. After treatment with PMA, the numbers of OTUs reduced by 4.4

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Author

Qidi Ren; Fangqiao Wei; Chao Yuan; Ce Zhu; Qian Zhang; Junkang Quan; Xiangyu Sun; Shuguo Zheng

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Dentistry (Health Sciences)