About this Medicine article
Meshwork arrangement of mitochondria-rich, Na+,K+-ATPase-rich cells in the saccular epithelium of rainbow trout (Oncorhynchus mykiss) inner ear by Yasuaki Takagi is a Medicine article available to read on EtoBox.
Background: Electrolyte composition of the teleost fish inner ear endolymph is characterized by a high potassium concentration. From the ultrastructural characteristics, the mitochondria-rich cells (MRCs) in the inner ear epithelium are suggested to regulate the ionic composition of the endolymph. Methods: In the present study, the ultrastructure of MRCs in the saccular epithelium of the rainbow trout (Onchorhynchus mykiss) was studied, and the immunocytochemical detection of Na 1 ,K 1 -ATPase, the key enzyme of the ion-transport, in the saccular epithelium was conducted. Electrolyte composition of the saccular endolymph was also determined. Results: Electron-microscopic observations revealed that MRCs located at the periphery of the sensory macula have numerous elongated mitochondria and a well-developed tubular system. Immunocytochemical detection of Na 1 ,K 1 -ATPase on paraffin sections showed that immunoreactive (ir-) cells were distributed specifically around the sensory macula. Judging from their shape, size, and localization, the Na 1 ,K 1 -ATPase ir-cells corresponded to the MRCs. The whole-mount immunocytochemistry using Na 1 ,K 1 -ATPase as a marker for the MRC revealed
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- Author
- Yasuaki Takagi
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0003-276X)
- Published
- 1997
- Language
- EN
- Field
- Medicine (Health Sciences)