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The Berlin-Buch respiration chamber for energy expenditure measurements by Anja Mähler; Till Schütte; Jochen Steiniger; Michael Boschmann is a Medicine article available to read on EtoBox.

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## Purpose We present a methodological overview of a respiration chamber at the Experimental and Clinical Research Center in Berlin, Germany. Since 2010, we investigated 750 healthy subjects and patients with various diseases. We routinely measure resting energy expenditure (REE), dietary-induced thermogenesis, and activity energy expenditure. ## Methods The chamber is a pull calorimeter with a total volume of 11,000 L. The majority of measurements is done with a flow rate of 120 L/min, yielding a favorable time constant of 1.53 h. The gas analysis system consists of two paramagnetic O~2~ sensors and two infrared CO~2~ sensors, one for incoming and one for outgoing air samples. O~2~ and CO~2~ sensors are calibrated simultaneously before each measurement with a 6 min calibration routine. To verify the accuracy of the whole the calorimetric system, it is validated every 2 weeks by 2 h acetone burning tests. ## Results Validation factors (calculated/measured) of 20 representative 2 h acetone burning tests were 1.03 ± 0.03 for \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \use

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Author

Anja Mähler; Till Schütte; Jochen Steiniger; Michael Boschmann

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN

Field

Medicine (Health Sciences)