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Altered expression of proteoglycan, collagen and growth factor genes in a TGF-Î21 stimulated genetic risk model for musculoskeletal soft tissue injuries by Willard, Kyle; Laguette, Mary-Jessica Nancy; Alves de Souza Rios, Leonardo; DâAlton, Caroline; Nel, Melissa; Prince, Sharon; Collins, Malcolm; September, Alison Victoria is a Medicine article available to read on EtoBox.

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## Objectives: To investigate the functional effect of implicated variants within bgn and col5a1 on gene expression of components of the extracellular matrix (ecm) in a tgf-β-stimulated risk model for musculoskeletal soft tissue injuries. ## Design: Experimental research, laboratory study. ## Methods: Skin biopsies were obtained from nine healthy participants with either a combined increased or reduced risk profile for col5a1 rs12722 c>t and bgn rs1126499 c>t - rs1042103 g>a, and primary fibroblast cell lines were established. total rna was extracted at baseline (10% fbs), after serum starvation (1% fbs) and tgf-β1 treatment (1% fbs, 10ng/ml tgf-1β). relative mrna levels of bgn, col5a1, dcn and vegfa was quantified using taqman® array pre-spotted plate assays (applied biosystems, foster city, ca, usa). ## Results: At baseline, the reduced risk group had 2.5, 1.9 and 2 fold increases (p<0.001) in relative bgn, col5a1 and vegfa mrna levels respectively. in the serum starved experiments, except for a significant 1.5 fold (p=0.017) increase in relative dcn mrna expression in the reduced risk group, similar observations were noted for the other three genes. after tgf-1β treatment, the r

Who reads Altered expression of proteoglycan, collagen and growth factor genes in a TGF-Î21 stimulated genetic risk model for musculoskeletal soft tissue injuries?

Author

Willard, Kyle; Laguette, Mary-Jessica Nancy; Alves de Souza Rios, Leonardo; DâAlton, Caroline; Nel, Melissa; Prince, Sharon; Collins, Malcolm; September, Alison Victoria

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1440-2440)

Published

2020

Language

EN

Field

Medicine (Health Sciences)