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Can I read Effects of Cellular Iron Deficiency on the Formation of Vascular Endothelial Growth Factor and Angiogenesis. on EtoBox?
Effects of Cellular Iron Deficiency on the Formation of Vascular Endothelial Growth Factor and Angiogenesis. by Jonathan Eckard; Jisen Dai; Jing Wu; Jinlong Jian; Qing Yang; Haobin Chen; Max Costa; Krystyna Frenkel; Xi Huang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Effects of Cellular Iron Deficiency on the Formation of Vascular Endothelial Growth Factor and Angiogenesis. about?
## Abstract ## Background Young women diagnosed with breast cancer are known to have a higher mortality rate from the disease than older patients. Specific risk factors leading to this poorer outcome have not been identified. In the present study, we hypothesized that iron deficiency, a common ailment in young women, contributes to the poor outcome by promoting the hypoxia inducible factor-1α (HIF-1α and vascular endothelial growth factor (VEGF) formation. This hypothesis was tested in an __in vitro__ cell culture model system. ## Results Human breast cancer MDA-MB-231 cells were transfected with transferrin receptor-1 (TfR1) shRNA to constitutively impair iron uptake. Cellular iron status was determined by a set of iron proteins and angiogenesis was evaluated by levels of VEGF in cells as well as by a mouse xenograft model. Significant decreases in ferritin with concomitant increases in VEGF were observed in TfR1 knockdown MDA-MB-231 cells when compared to the parental cells. TfR1 shRNA transfectants also evoked a stronger angiogenic response after the cells were injected subcutaneously into nude mice. The molecular mechanism appears that cellular iron deficiency elevates VEGF for
Who reads Effects of Cellular Iron Deficiency on the Formation of Vascular Endothelial Growth Factor and Angiogenesis.?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Jonathan Eckard; Jisen Dai; Jing Wu; Jinlong Jian; Qing Yang; Haobin Chen; Max Costa; Krystyna Frenkel; Xi Huang
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1475-2867)
- Published
- 2010
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)