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High-throughput On-line Solid-phase Extraction–liquid Chromatography–tandem Mass Spectrometry Method for the Simultaneous Analysis of 14 Antidepressants and Their Metabolites in Plasma by Ana de Castro; Maria del Mar Ramírez Fernandez; Marleen Laloup; Nele Samyn; Gert De Boeck; Michelle Wood; Viviane Maes; Manuel López-Rivadulla is a Chemistry article available to read on EtoBox.
What is High-throughput On-line Solid-phase Extraction–liquid Chromatography–tandem Mass Spectrometry Method for the Simultaneous Analysis of 14 Antidepressants and Their Metabolites in Plasma about?
A rapid, sensitive and fully automated on-line solid-phase extraction-liquid chromatography-tandem mass spectrometry (SPE-LC-MS/MS) method was developed and validated for the direct analysis of 14 antidepressants and their metabolites in plasma. Integration of the sample extraction and LC separation into a single system permitted direct injection of the plasma without prior sample pre-treatment. The applied gradient ensured the elution of all the examined drugs within 14 min and produced chromatographic peaks of acceptable symmetry. The total process time was 20 min and only 50 L of plasma was required. Selectivity of the method was achieved by a combination of retention time and two precursor-product ion transitions for the non-deuterated compounds. The use of SPE was demonstrated to be highly effective and led to significant decreases in the interferences present in the matrix. Extraction was found to be both reproducible and efficient with recoveries >99% for all the analytes. The method showed excellent intra-assay and inter-assay precision (relative standard deviation (RSD) and bias 0.99 over the range investigated (10-1000 g/L). Limits of quantification (LOQs) were estimate
Who reads High-throughput On-line Solid-phase Extraction–liquid Chromatography–tandem Mass Spectrometry Method for the Simultaneous Analysis of 14 Antidepressants and Their Metabolites in Plasma?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Ana de Castro; Maria del Mar Ramírez Fernandez; Marleen Laloup; Nele Samyn; Gert De Boeck; Michelle Wood; Viviane Maes; Manuel López-Rivadulla
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2007
- Language
- EN
- Field
- Chemistry (Physical Sciences)