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Biosynthesis of Nondigestible Galactose-Containing Hetero-oligosaccharides by Lactobacillus plantarum WCFS1 MelA α-Galactosidase by Paloma Delgado-Fernandez; Blanca de las Rivas; Rosario Muñoz; María Luisa Jimeno; Elisa G. Doyagüez; Nieves Corzo; F. Javier Moreno is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Biosynthesis of Nondigestible Galactose-Containing Hetero-oligosaccharides by Lactobacillus plantarum WCFS1 MelA α-Galactosidase about?
This work describes the high capacity of MelA α-galactosidase from Lactobacillus plantarum WCFS1 to transfer galactosyl residues from melibiose to the C6-hydroxyl group of disaccharide-acceptors with β-linkages (lactulose, lactose, and cellobiose) or α-linkages (isomaltulose and isomaltose) to produce novel galactose-containing hetero-oligosaccharides (HOS). A comprehensive nuclear magnetic resonance characterization of the transfer products derived from melibiose:lactulose reaction mixtures revealed the biosynthesis of α-D-galactopyranosyl-(1 → 6)-β-D-galactopyranosyl-(1 → 4)-β-D-fructose as the main component as well as the presence of α-D-galactopyranosyl-Melibiose-derived αgalactooligosaccharides (α-GOS), manninotriose and verbascotetraose, were also simultaneously synthesized. An in vitro assessment of the intestinal digestibility of the novel biosynthesized HOS revealed a high resistance of α-galactosides derived from lactulose, lactose, cellobiose, and isomaltulose. According to the evidence gathered for conventional α-GOS and certain disaccharides used as acceptors in this work, these novel nondigestible α-galactosides could be potential candidates to selectively modulate t
Who reads Biosynthesis of Nondigestible Galactose-Containing Hetero-oligosaccharides by Lactobacillus plantarum WCFS1 MelA α-Galactosidase?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Paloma Delgado-Fernandez; Blanca de las Rivas; Rosario Muñoz; María Luisa Jimeno; Elisa G. Doyagüez; Nieves Corzo; F. Javier Moreno
- Publisher
- American Chemical Society (ACS)
- Published
- 2021
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)