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Streptococcus Agalactiae Amylomaltase Offers Insight Into the Transglycosylation Mechanism and the Molecular Basis of Thermostability Among Amylomaltases by Tumhom, Suthipapun (author);Nimpiboon, Pitchanan (author);Wangkanont, Kittikhun (author);Pongsawasdi, Piamsook (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Streptococcus Agalactiae Amylomaltase Offers Insight Into the Transglycosylation Mechanism and the Molecular Basis of Thermostability Among Amylomaltases about?
## Abstract Amylomaltase (AM) catalyzes transglycosylation of starch to form linear or cyclic oligosaccharides with potential applications in biotechnology and industry. In the present work, a novel AM from the mesophilic bacterium Streptococcus agalactiae (SaAM), with 18–49% sequence identity to previously reported AMs, was characterized. Cyclization and disproportionation activities were observed with the optimum temperature of 30 °C and 40 °C, respectively. Structural determination of SaAM, the first crystal structure of small AMs from the mesophiles, revealed a glycosyl-enzyme intermediate derived from acarbose and a second acarbose molecule attacking the intermediate. This pre-transglycosylation conformation has never been before observed in AMs. Structural analysis suggests that thermostability in AMs might be mainly caused by an increase in salt bridges since SaAM has a lower number of salt bridges compared with AMs from the thermophiles. Increase in thermostability by mutation was performed. C446 was substituted with A/S/P. C446A showed higher activities and higher k~cat~/K~m~ values for starch in comparison to the WT enzyme. C446S exhibited a 5 °C increase in optimum tempe
Who reads Streptococcus Agalactiae Amylomaltase Offers Insight Into the Transglycosylation Mechanism and the Molecular Basis of Thermostability Among Amylomaltases?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Tumhom, Suthipapun (author);Nimpiboon, Pitchanan (author);Wangkanont, Kittikhun (author);Pongsawasdi, Piamsook (author)
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)