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This document presents the characterization and validation of a novel group of Type V, Class 2 CRISPR nucleases, termed Cms1, identified from Microgenomates and Smithella bacterial species. The Cms1 nucleases are smaller, do not require a tracrRNA, and have distinct protospacer-adjacent motif site requirements, demonstrating efficient genome editing capabilities in rice. This discovery expands the Type V CRISPR toolbox, offering diverse options for researchers in genome editing applications.

Author

dukepanda1

Language

EN