About this Neuroscience article

Functional Characterization of the Murine Serotonin Transporter Gene Promoter in Serotonergic Raphe Neurons by Armin Heils; Christine Wichems; Rainald Mössner; Susanne Petri; Katharina Glatz; Dietmar Bengel; Dennis L. Murphy; Klaus‐Peter Lesch is a Neuroscience article available to read on EtoBox.

Abstract: We have isolated and characterized the 5′‐flanking regulatory region of the murine serotonin 5‐HT transporter (5‐HTT) gene. A TATA‐like motif and several potential binding sites for transcription factors, including two AP1, several AP2 and AP4 binding sites, CCAAT and GC boxes (SP1 binding sites), a nuclear factor‐κB, and a cyclic AMP response element‐like motif, are present in the 5′‐flanking region. A ∼2.2‐kb fragment (−2,143 to +51 with respect to the transcription start site), which had been fused to the luciferase reporter gene and transiently expressed in a 5‐HTT‐expressing cell line and in serotonergic raphe neurons derived from embryonic rat brain‐stem, displayed both constitutive and inducible promoter activity. Functional promoter mapping revealed two clusters of activating elements from bp −82 to −527 and bp −1,001 to −1,937. A cell/neuron‐selective silencer element(s) is contained between bp −294 and −527. Our findings suggest that (1) the murine 5‐HTT gene promoter is active in serotonergic raphe neurons but significantly repressed in neuronal cells from frontal cortex that do not express 5‐HTT, (2) the information contained within ∼0.5 kb of the 5′‐flanking

Author

Armin Heils; Christine Wichems; Rainald Mössner; Susanne Petri; Katharina Glatz; Dietmar Bengel; Dennis L. Murphy; Klaus‐Peter Lesch

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1998

Language

EN

Field

Neuroscience (Life Sciences)