Can I read Protein Delivery by PTDs/CPPs on EtoBox?
Protein Delivery by PTDs/CPPs by Caroline Palm-Apergi; Steven F. Dowdy is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Protein Delivery by PTDs/CPPs about?
The ability to deliver or transduce proteins into cells allows for the manipulation of cell biology in culture, preclinical models, and potentially human disease. Fusion proteins containing the TAT peptide transduction domain (PTD), also known as cell-penetrating peptide (CPP), allow for delivery of a wide variety of proteins, including enzymes, transcription factors, tumor suppressor proteins, and many more. TAT-fusion proteins are generated cloning in-frame into the pTAT-HA plasmid, then transformed into E. coli for expression, and purified by the 6-His affinity tag over Ni-NTA column, followed by a final IEX FPLC purification step.
Who reads Protein Delivery by PTDs/CPPs?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Caroline Palm-Apergi; Steven F. Dowdy
- Publisher
- Springer US : Imprint : Humana
- Published
- 2021
- Language
- EN
- ISBN
- 9781071617519
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)