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Can I read Mitotic control of RUNX2 phosphorylation by both CDK1/cyclin B kinase and PP1/PP2A phosphatase in osteoblastic cells on EtoBox?
Mitotic control of RUNX2 phosphorylation by both CDK1/cyclin B kinase and PP1/PP2A phosphatase in osteoblastic cells by Arun Rajgopal; Daniel W. Young; Khawaja A. Mujeeb; Janet L. Stein; Jane B. Lian; Andre J. van Wijnen; Gary S. Stein is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
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## Abstract Skeletal development and osteoblast maturation require the phenotype promoting activity of the transcription factor RUNX2, which controls both cell growth and differentiation in osteoblasts. We have recently shown that in actively proliferating cells RUNX2 regulates the expression of specific target genes as cells enter and exit mitosis. In this study, we addressed whether post‐translational modifications of RUNX2 control its activity during mitotic exit. Western blot analysis of proteins from osteoblastic Saos‐2 cells released from mitotic inhibition into early G~1~ show a phosphatase‐sensitive shift in the mobility of RUNX2 in SDS gels. The slowly migrating hyper‐phosphorylated form of RUNX2 is immunoreactive with a CDK related phospho‐antibody (MPM2) only in mitotic cells and is converted into a faster migrating hypo‐phosphorylated RUNX2 when cells complete mitosis. This conversion is inhibited by okadaic acid, an inhibitor of protein phosphatases 1 and 2 (PP1 and PP2A), but not by deltamethrin which blocks PP2B phosphatase. Mitotic phosphorylation of RUNX2 is sensitive to the CDK inhibitors roscovitine and olomoucine. Furthermore, RUNX2 can directly interact with CD
Who reads Mitotic control of RUNX2 phosphorylation by both CDK1/cyclin B kinase and PP1/PP2A phosphatase in osteoblastic cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Arun Rajgopal; Daniel W. Young; Khawaja A. Mujeeb; Janet L. Stein; Jane B. Lian; Andre J. van Wijnen; Gary S. Stein
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)
- Published
- 2006
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)