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Effects of Lipopolysaccharide Exposure on the Inflammatory Response, Butyrate Flux, and Metabolic Function of the Ruminal Epithelium Using an Ex Vivo Model by Kent-Dennis, C.; Penner, G.B. is a Agricultural and Biological Sciences article available to read on EtoBox.

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Acidotic conditions in the rumen have been associated with compromised barrier function of the ruminal epithelium and translocation of microbe-associated molecular patterns (MAMP) such as lipopolysaccharide (LPS). Interaction of MAMP with the ruminal epithelium may also induce a local proinflammatory response. The aim of this study was to evaluate the potential proinflammatory response of the ruminal epithelium following LPS exposure in Ussing chambers, to investigate whether LPS exposure affects the flux and metabolism of butyrate. Ruminal epithelial tissue from 9 Holstein bull calves were mounted into Ussing chambers and exposed to 0, 10,000, 50,000, or 200,000 endotoxin units (EU)/mL LPS for a duration of 5 h. Radiolabeled C-butyrate (15 mM) was added to the mucosal buffer to assess the mucosal-to-serosal flux of C-butyrate. Additional Ussing chambers, without radioisotope, were exposed to either 0 or 200,000 EU/mL LPS and were used to measure the release of β-hydroxybutyrate (BHB) and IL1B into the buffer, and to collect epithelial tissue for analysis of gene expression. Genes associated with inflammation (TNF, IL1B, CXCL8, PTGS2, TGFB1, TLR2, TLR4), nutrient transport (MCT1, M

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Author

Kent-Dennis, C.; Penner, G.B.

Publisher

American Dairy Science Association; Elsevier Ltd; Elsevier BV (ISSN 0022-0302)

Published

2020

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)