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Subcellular localization of ammonium transporters in Dictyostelium discoideum by Janet H Kirsten; Yanhua Xiong; Carter T Davis; Charles K Singleton is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract ## Background With the exception of vertebrates, most organisms have plasma membrane associated ammonium transporters which primarily serve to import a source of nitrogen for nutritional purposes. __Dictyostelium discoideum__ has three ammonium transporters, Amts A, B and C. Our present work used fluorescent fusion proteins to determine the cellular localization of the Amts and tested the hypothesis that the transporters mediate removal of ammonia generated endogenously from the elevated protein catabolism common to many protists. ## Results Using RFP and YFP fusion constructs driven by the actin 15 promoter, we found that the three ammonium transporters were localized on the plasma membrane and on the membranes of subcellular organelles. AmtA and AmtB were localized on the membranes of endolysosomes and phagosomes, with AmtB further localized on the membranes of contractile vacuoles. AmtC also was localized on subcellular organelles when it was stabilized by coexpression with either the AmtA or AmtB fusion transporter. The three ammonium transporters exported ammonia linearly with regard to time during the first 18 hours of the developmental program as revealed by redu

Who reads Subcellular localization of ammonium transporters in Dictyostelium discoideum?

Author

Janet H Kirsten; Yanhua Xiong; Carter T Davis; Charles K Singleton

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, [2000]-2018.; Springer Science and Business Media LLC (ISSN 1471-2121)

Published

2008

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)