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Gene mutation of transforming growth factor beta1 type II receptor in hepatocellular carcinoma by Keiko Furuta; Sachiko Misao; Kiyoshi Takahashi; Tsuneaki Tagaya; Yoshitaka Fukuzawa; Tetsuya Ishikawa; Kentaro Yoshioka; Shinichi Kakumu is a Medicine article available to read on EtoBox.

What is Gene mutation of transforming growth factor beta1 type II receptor in hepatocellular carcinoma about?

Alteration of transforming growth factor beta1 (TGF-beta1) type II receptor (RII) appears to cause unresponsiveness to TGF-beta1 in tumorigenic cells. Defect in the mononucleotide repeat sequence, i.e., poly A region of TGF-beta1RII gene has been reported to be related to replication error-positive cancer cells. We examined if there is any TGF-beta1RII mutation in a coding microsatellite in hepatocellular carcinoma (HCC). Genomic DNAs were extracted from formalin-fixed, paraffin-embedded liver tissues obtained at surgery or autopsy in 3 normal individuals and 96 patients with hepatitis C virus-induced chronic liver disease; 3 with chronic hepatitis, 20 with liver cirrhosis and 73 with HCC. The DNA was PCR-amplified at 2 segments of TGF-beta1RII: poly A region which includes the (A)10 microsatellite sequence, and poly GT region. PCR products were directly sequenced. DNA from normal and patients with chronic liver disease contained the 10 wild-type adenines but 3 cases with liver cirrhosis in whom there were only 9 adenines within poly A tract. This microdeletion of one A resulted in a frameshift and truncated a predicted length of amino acids. In HCC lesions, the same deletion was n

Who reads Gene mutation of transforming growth factor beta1 type II receptor in hepatocellular carcinoma?

Author

Keiko Furuta; Sachiko Misao; Kiyoshi Takahashi; Tsuneaki Tagaya; Yoshitaka Fukuzawa; Tetsuya Ishikawa; Kentaro Yoshioka; Shinichi Kakumu

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

1999

Language

EN

Field

Medicine (Health Sciences)