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Development of a Holder to Avoid Handling Human Crystalline Lenses in Biological Assays by Fernanda O. Duarte; Mauro Masili; Márcio M. Mello; Sidney J. Faria-e-Sousa; Liliane Ventura is a Medicine article available to read on EtoBox.

What is Development of a Holder to Avoid Handling Human Crystalline Lenses in Biological Assays about?

Purpose The importance of this study is to develop handling strategies for human lens studies, aiming at eye health. The aim of this study was to develop a lens holder to facilitate handling lenses having minimal contact with different surfaces while developing experimental protocols. Methods We devised a dissection technique to harvest the lens together with the iris and ciliary body, avoiding any contact with the lens. We also made a specific holder for the lens. Five human lenses were used and preserved in TC199 solution at 4 °C. To test the practicality of the new tool, we weighed the lenses and performed transmission spectroscopy to evaluate the holder's interference during measurements. ## Results The lens holder consists of a pair of anodized plaques with eight pins attached to one of the plaques. The lens is accommodated in the holder by attaching the ciliary muscle to the pins. The holder allows the lens to float inside it, preventing it from deforming, and keeping it intact during the experiment. Overall, the lens holder showed suitable handling in repeated measurement experiments of spectral transmission and daily measurement of the lens mass. In both analyses, the lense

Who reads Development of a Holder to Avoid Handling Human Crystalline Lenses in Biological Assays?

Author

Fernanda O. Duarte; Mauro Masili; Márcio M. Mello; Sidney J. Faria-e-Sousa; Liliane Ventura

Publisher

Springer Science and Business Media LLC

Published

2021

Language

EN

Field

Medicine (Health Sciences)