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Biochemical and molecular characterization of NADP-glutamate dehydrogenase from the ectomycorrhizal fungus Tuber borchii by Luciana Vallorani; Emanuela Polidori; Cinzia Sacconi; Deborah Agostini; Raffaella Pierleoni; Giovanni Piccoli; Sabrina Zeppa; Vilberto Stocchi is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Biochemical and molecular characterization of NADP-glutamate dehydrogenase from the ectomycorrhizal fungus Tuber borchii about?
## Summary • NADP‐glutamate dehydrogenase (NADP‐GDH) from __Tuber borchii__ was purified and the corresponding gene was cloned in order to elucidate the physiological role of the enzyme in this ectomycorrhizal fungus. • NADP‐GDH was purified using an anion‐exchange column followed by affinity chromatography. The complete gene was cloned from a 30‐d‐old‐mycelium cDNA library and characterized. • __T. borchii__ NADP‐GDH appears to be physically and kinetically similar to those from other fungi and the deduced amino acid sequence of the __gdh__ gene showed a significant similarity to other fungal NADP‐dependent GDHs. Biochemical and Northern blotting analyses carried out with mycelia grown on different nitrogen sources clearly showed that the regulation of __T. borchii__ NADP‐GDH in response to different nitrogen sources was markedly different from the responses of the NADP‐GDHs of other ascomycetes. Northern blotting analyses highlighted that the __gdh__ gene was also expressed in the symbiotic phase. • The biochemical and molecular data suggest that the fungal NADP‐GDH contributes to the primary nitrogen metabolism in the ectomycorrhizal tissues.
Who reads Biochemical and molecular characterization of NADP-glutamate dehydrogenase from the ectomycorrhizal fungus Tuber borchii?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Luciana Vallorani; Emanuela Polidori; Cinzia Sacconi; Deborah Agostini; Raffaella Pierleoni; Giovanni Piccoli; Sabrina Zeppa; Vilberto Stocchi
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Cambridge University Press (CUP); Wiley (ISSN 0028-646X)
- Published
- 2002
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)