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Effects of 810 nm laser irradiation on in vitro growth of bacteria: Comparison of continuous wave and frequency modulated light by Ethne L. Nussbaum; Lothar Lilge; Tony Mazzulli is a Medicine article available to read on EtoBox.

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## Abstract ## Background and Objectives Low intensity laser therapy may modify growth of wound bacteria, which could affect wound healing. This study compares the effects on bacteria of 810 nm laser using various delivery modes (continuous wave or frequency modulated light at 26, 292, 1,000, or 3,800 Hz). ## Study Design/Materials and Methods __Staphylococcus__ (__S.__) __aureus__, __Escherichia__ (__E.__) __coli__, and __Pseudomonas__ (__P.__) __aeruginosa__ were plated on agar and then irradiated (0.015 W/cm^2^; 1–50 J/cm^2^) or used as controls (sham irradiated); growth was examined after 20 hours of incubation post exposure. ## Results There were interactions of species and modulation frequency in the overall effects of irradiation (__P__ = 0.0001), and in the radiant exposure mediated effects (__P__ = 0.0001); thus individual frequencies and each bacterium were analysed separately. Bacteria increased following 3,800 Hz (__P__ = 0.0001) and 1,000 Hz (__P__ = 0.0001) pulsed irradiation; at particular radiant exposures __P. aeruginosa__ proliferated significantly more than other bacteria. Pulsed laser at 292 and 26 Hz also produced species‐dependent effects (__P__ = 0.0001; __P_

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Author

Ethne L. Nussbaum; Lothar Lilge; Tony Mazzulli

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0196-8092)

Published

2002

Language

EN

Field

Medicine (Health Sciences)