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Can I read Improving Cell-free Glycoprotein Synthesis by Characterizing and Enriching Native Membrane Vesicles on EtoBox?
Improving Cell-free Glycoprotein Synthesis by Characterizing and Enriching Native Membrane Vesicles by Hershewe, Jasmine M. (author);Warfel, Katherine F. (author);Iyer, Shaelyn M. (author);Peruzzi, Justin A. (author);Sullivan, Claretta J. (author);Roth, Eric W. (author);DeLisa, Matthew P. (author);Kamat, Neha P. (author);Jewett, Michael C. (author) is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Improving Cell-free Glycoprotein Synthesis by Characterizing and Enriching Native Membrane Vesicles about?
Cell-free gene expression (CFE) systems from crude cellular extracts have attracted much attention for biomanufacturing and synthetic biology. However, activating membrane-dependent functionality of cell-derived vesicles in bacterial CFE systems has been limited. Here, we address this limitation by characterizing native membrane vesicles in __Escherichia coli-__based CFE extracts and describing methods to enrich vesicles with heterologous, membrane-bound machinery. As a model, we focus on bacterial glycoengineering. We first use multiple, orthogonal techniques to characterize vesicles and show how extract processing methods can be used to increase concentrations of membrane vesicles in CFE systems. Then, we show that extracts enriched in vesicle number also display enhanced concentrations of heterologous membrane protein cargo. Finally, we apply our methods to enrich membrane-bound oligosaccharyltransferases and lipid-linked oligosaccharides for improving cell-free __N-__linked and __O__-linked glycoprotein synthesis. We anticipate that these methods will facilitate on-demand glycoprotein production and enable new CFE systems with membrane-associated activities.
Who reads Improving Cell-free Glycoprotein Synthesis by Characterizing and Enriching Native Membrane Vesicles?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Hershewe, Jasmine M. (author);Warfel, Katherine F. (author);Iyer, Shaelyn M. (author);Peruzzi, Justin A. (author);Sullivan, Claretta J. (author);Roth, Eric W. (author);DeLisa, Matthew P. (author);Kamat, Neha P. (author);Jewett, Michael C. (author)
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)