Can I read An effective and rapid method for RNA preparation from non-conventional yeast species on EtoBox?

An effective and rapid method for RNA preparation from non-conventional yeast species by Dong Wook Lee; Chang Pyo Hong; Hyun Ah Kang is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is An effective and rapid method for RNA preparation from non-conventional yeast species about?

The increased use of high-throughput RNA-based analysis has spurred the demand for rapid and simple preparation of high quality RNA. RNA preparation from non-conventional yeasts having diverse cell wall and morphological characteristics is often inefficient using current methods adapted for the model yeast, Saccharomyces cerevisiae. We report a simple RNA preparation method based on glass bead-mediated breakage in a formamide/EDTA solution. High quality RNA is generated within 15 min from various non-conventional yeasts species. The obtained RNA can be directly used for experimentation without further RNA purification and buffer exchange.

Who reads An effective and rapid method for RNA preparation from non-conventional yeast species?

Author

Dong Wook Lee; Chang Pyo Hong; Hyun Ah Kang

Published

2019

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)