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Can I read Expression, purification, crystallization and preliminary X-ray analysis of α-l-arabinofuranosidase B from Aspergillus kawachii on EtoBox?
Expression, purification, crystallization and preliminary X-ray analysis of α-l-arabinofuranosidase B from Aspergillus kawachii by Akimasa Miyanaga; Takuya Koseki; Hiroshi Matsuzawa; Takayoshi Wakagi; Hirofumi Shoun; Shinya Fushinobu is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Expression, purification, crystallization and preliminary X-ray analysis of α-l-arabinofuranosidase B from Aspergillus kawachii about?
l-Arabinofuranosidase (EC 3.2.1.55) is one of the hemicellulases that cleave the glycosidic bonds between l-arabinofuranoside side chains and various oligosaccharides. In this study, the ®rst crystallization and preliminary X-ray analysis of -l-arabinofuranosidase B from Aspergillus kawachii IFO4308 (AkAbfB), a family 54 glycoside hydrolase, is described. Recombinant AkAbfB was expressed in Escherichia coli and Pichia pastoris. The native crystals of recombinant AkAbfB produced by P. pastoris belong to the orthorhombic space group P2 1 2 1 2 1 (unit-cell parameters a = 39.5, b = 98.2, c = 144.0 A Ê ) and diffracted X-rays to a resolution of 1.82 A Ê .
Who reads Expression, purification, crystallization and preliminary X-ray analysis of α-l-arabinofuranosidase B from Aspergillus kawachii?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Akimasa Miyanaga; Takuya Koseki; Hiroshi Matsuzawa; Takayoshi Wakagi; Hirofumi Shoun; Shinya Fushinobu
- Publisher
- International Union of Crystallography; Blackwell Publishing Inc.; International Union of Crystallography (IUCr) (ISSN 0907-4449)
- Published
- 2004
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)